| 质粒名称: | pIB184 (EM) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 90194 |
| 存储实验室: | Indranil Biswas |
|
载体骨架基本信息 |
|
| 载体名称: | pAMB1 (pORI23) |
| 载体出品公司: | Philippe Moreillon (PMID 10816506) |
| 空载体大小: | 6000bp |
| 完整质粒大小: | 6086bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | Erythromycin, 200 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 无 |
| 标签/融合蛋白: |
None (N terminal on backbone)
None (C terminal on backbone)
|
|
文献引用方法 |
|
| 材料和方法部分: | pIB184 (EM) was a gift from Indranil Biswas (Addgene plasmid # 90194 ; http://n2t.net/addgene:90194 ; RRID:Addgene_90194) |
| 参考文献部分: | Shuttle expression plasmids for genetic studies in Streptococcus mutans. Biswas I, Jha JK, Fromm N. Microbiology. 2008 Aug;154(Pt 8):2275-82. doi: 10.1099/mic.0.2008/019265-0. 10.1099/mic.0.2008/019265-0 PubMed 18667560 |
买家导航
pIB184 (EM)载体质粒基本信息
pIB184 (EM)质粒图谱载体图谱和pIB184 (EM)载体序列质粒序列多克隆位点信息
质粒图谱.png)
pIB184 (EM)质粒载体简介
pIB184 (EM)质粒序列载体序列
LOCUS pIB184 (EM) 6086 bp DNA circular SYN 14-APR-2025
DEFINITION E. coli - Streptococci shuttle plasmid for gene expression in
streptococci with P23 promoter.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6086)
AUTHORS Biswas I, Jha JK, Fromm N
TITLE Shuttle expression plasmids for genetic studies in Streptococcus
mutans.
JOURNAL Microbiology. 2008 Aug;154(Pt 8):2275-82. doi:
10.1099/mic.0.2008/019265-0.
PUBMED 18667560
REFERENCE 2 (bases 1 to 6086)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Microbiology."; date: "2008-08"; volume: "154(Pt 8):2275-82. doi";
pages: " 10.1099/mic.0.2008/019265-0"
COMMENT Sequence Label: pIB184 (EM)
FEATURES Location/Qualifiers
source 1..6086
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 5233..5821
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5722..5741
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5975..5992
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 cgaaaagccc tgacaaccct tgttcctaaa aaggaataag cgttcggtca gtaaataata
61 gaaataaaaa atcagaccta agactgatga caaaaagaga aaattttgat aaaatagtct
121 tagaattaaa ttaaaaaggg aggccaaata taatgaaaaa tatgaatgac aatgatgttg
181 gatccccgcg gtacccggga attctagagc tcgagatcta tcgataagct taagtgatta
241 gtcaaagaat ggtgatgaca attgtaaatt ctatttaatc actttgacta gcaaatacta
301 acaacaagac acacacacca aaaatcaaaa attcactact tttagttaaa aaccacgtaa
361 ccacaagaac taatccaatc catgtaatcg ggttcttcaa atatttctcc aagattttcc
421 tcctctaata tgctcaactt aaatgaccta ttcaataaat ctattatgct gctaaatagt
481 ttataggaca aataagtata ctctaatgac ctataaaaga tagaaaatta aaaaatcaag
541 tgttcgcttc gctctcactg cccctcgacg ttttagtagc ctttccctca cttcgttcag
601 tccaagccaa ctaaaagttt tcgggctact ctctccttct ccccctaata attaattaaa
661 atcttactct gtatatttct gctaatcatt cgctaaacag caaagaaaaa acaaacacgt
721 atcatagata taaatgtaat ggcatagtgc gggttttatt ttcagcctgt atcatagcta
781 aacaaatcga gttgtgtgtc cgttttaggg cgttctgcta gcttgtttaa agtctcttga
841 atgaatgtat gctctaagtc aaaagaattt gtcagcgcct ttatatagct ttctttttct
901 tcttttttta ctttaatgat cgatagcaac aatgatttaa cactagcaag ttgaatgcca
961 ccatttcttc ctggtttaat cttaaagaaa atttcctgat tcgccttcag taccttcagc
1021 aatttatcta atgtccgttc aggaatgcct agcacttctc taatctcttt tttggtcgtc
1081 actaaataag gcttgtatac atcgcttttt tcgctaatat aagccattaa atcttctttc
1141 cattctgaca aatgaacacg ttgacgttcg cttctttttt tcttgaattt aaaccaccct
1201 tgacggacaa ataaatcttt actggttaaa tcacttgata cccaagcttt gcaaagaatg
1261 gtaatgtatt ccctattagc cccttgatag ttttctgaat aggcacttct aacaattttg
1321 attacttctt tttcttctaa gggttgatct aatcgattat taaactcaaa catattatat
1381 tcgcacgttt cgattgaata gcctgaacta aagtaggcta aagagagggt aaacatgacg
1441 ttattacgcc ctattaaacc cttttctcct gaaaatttcg tttcgtgcaa taagagatta
1501 aaccagggtt catctacttg ttttttgcct tctgtaccgc ttaaaaccgt tagacttgaa
1561 cgagtaaagc ccttattatc tgtttgtttg aaagaccaat cttgccattc tttgaaagaa
1621 taacggtaat taggatcaaa aaattctaca ttgtccgttc ttggtatgcg agcaatacca
1681 aaatgattac acgttagatc aactggcaaa gactttccaa aatattctcg gatattttgc
1741 gaaattattt tggctgcttt gacagattta aattctgatt ttgaagtcac atagactggc
1801 gtttctaaaa caaaatatgc ttgataacct ttatcagatt tgataatcat agtaggcata
1861 aaacctaaat caatagcggt tgttaaaata tcgcttgctg aaatagtttc ttttgccgtg
1921 tgaatatcaa aatcaataaa gaaggtattg atttgtctta aattgttttc agaatgtcct
1981 ttcgtgtatg aacggttttc gtctgcatac gttccataac gataaacgtt gggtgtccaa
2041 tgtgtaaatg tatcttgatt ttcttgaatc gcttcctcgg aagtcagaac aacaccacga
2101 ccgccaatca tgcttgattt tgagcgatac gcaaaaatag cccctttgct tttacctggc
2161 ttggtagtga ttgagcgaat tttactattt ttaaatttgt actttaacaa gccgtcatga
2221 agcacagttt ctacaacaaa agggatattc attcagctgt tctcctttcc tataaatcct
2281 ataaaatagg ttgtttaatt aacttggttt gctttttcat tcaactgttt caatattgca
2341 tgttttgaaa aagatttttt tcctttataa gtcaattttt ttccactaat cgaataaatt
2401 attttgttat tttctattaa cttatatata taatcttccc cctccgaaga aaaatactta
2461 tctgattttg tttctaagta gatatttctc ttttctaact ctttcttaaa cgtttctagt
2521 gtatagatat ttgctaattt tcttatctcc aataaactat tttttatata agttttacat
2581 tcatcatgat tcatacaaac tccaccttct ataaatgaat acaaaaaaag caatcaaacg
2641 atttccgatt gattgcttaa caattcttaa attcagtagc ttagatactt gaaaactctc
2701 tgatttccct atataatgat agtacggtta tataccgtct tcaaacaaag ttaattaaat
2761 aacttcttac gagggaagag ttcatctgac taactgataa gcgttggttt ggcaatctta
2821 tcgggctatg catttataaa atgtcgtcaa acattttata aatgtgtcat ggctcttttt
2881 tcgtttctat tcagttcgtt gtttcgttat atctagtata ccgcttttaa aaaaaataag
2941 caacgatttc gtgcattatt cacacgaagt cattgctttt ttcttcttcc atttctaaat
3001 ccaatgttac ttgttctgat tctgtttctg gttctggttc tgttggctca tttgggatta
3061 aatccactac tagcgttgag ttagttaact ttgcaatttg ttctagtgtt tttatggttg
3121 gatctgattt tcctgattct attcgtgaat aatttgatct actcatttct aattcttggg
3181 gtaccgccag catttcggaa aaaaaccacg ctaaggattt tttctataaa aagagccgtt
3241 atattaagaa taaaacggct cttttatacg taaaggacgt aaattcattt gcccagtgtc
3301 atgtaatcct tcaaatttgt attctccaag aaaattgata tgttcccatc ctaacggcca
3361 cgcatatggc attaaatctt ctctaaattc tcctcttgct tttaattctt ctacggcttt
3421 ttccatatat acagtgttcc acacacttat agcgttaata attatgttta gtgcactagc
3481 tctttgtaac tggtcttgga gagcacgttc tctaaattct ccacgttgtc caaaaaatat
3541 aattctagct aatgcattga ttgcttctcc tttatttaaa cctttttgaa cccgtctcct
3601 tacggcttta ttagatatgt aatccagcgt aaagagggtt ttctcgattc gtcccatttc
3661 tccaagtgct gttgcgagtt tattttgtct tgcatatgat ccgagcttcc ccatgataag
3721 agcgctaggg acctctttag ctccttggaa gctgtcagta gtatacctaa taatttatct
3781 acattccctt tagtaacgtg taactttcca aatttacaaa agcgactcat agaattattt
3841 cctcccgtta aataatagat aactattaaa aatagacaat acttgctcat aagtaacggt
3901 acttaaattg tttactttgg cgtgtttcat tgcttgatga aactgatttt tagtaaacag
3961 ttgacgatat tctcgattga cccattttga aacaaagtac gtatatagct tccaatattt
4021 atctggaaca tctgtggtat ggcgggtaag ttttattaag acactgttta cttttggttt
4081 aggatgaaag cattccgctg gcagcttaag caattgctga atcgagactt gagtgtgcaa
4141 gagcaaccct agtgttcggt gaatatccaa ggtacgcttg tagaatcctt cttcaacaat
4201 cagatagatg tcagacgcat ggctttcaaa aaccactttt ttaataattt gtgtgcttaa
4261 atggtaagga atactcccaa caattttata cctctgtttg ttagggaatt gaaactgtag
4321 aatatcttgg tgaattaaag tgacacgagt attcagtttt aatttttctg acgataagtt
4381 gaatagatga ctgtctaatt caatagacgt tacctgttta cttattttag ccagtttcgt
4441 cgttaaatgc cctttacctg ttccaatttc gtaaacggta tcggtttctt ttaaattcaa
4501 ttgttttatt atttggttga gtactttttc actcgttaaa aagttttgag aatattttat
4561 atttttgttc atgtaatcac tccttcttaa ttacaaattt ttagcatcta atttaacttc
4621 aattcctatt atacaaaatt ttaagatact gcactatcaa cacactctta agtttgcttc
4681 taagtcttat ttccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta
4741 tgatatggtg caagtcagca cgaacacgaa ccgtcttatc tcccattata tctttttttg
4801 cactgattgg tgtatcattt cgtttttctt tttgtgcgct tcttgataaa agggatagta
4861 attcattcct ggttgcaaat tttgaaaacc gctacggatc acatcttttt ctaaactatt
4921 gatccatagt cttttatacg ttttatcttt agaaaaggca tttgctttat gaatgatcga
4981 ccaggcaatg ttttcgcctt ctctgtcgct atctgttgcg acaatgattg tatttgcttg
5041 ttttaaaagt tcagcaacaa ttttaaactg ctttttttta tctgttgcca cttcaaaatc
5101 gtatcgattc tagatttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
5161 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
5221 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
5281 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
5341 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
5401 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
5461 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
5521 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
5581 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
5641 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
5701 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
5761 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
5821 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
5881 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
5941 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
6001 agagcgtcta gaatcgataa gcttggctgc aggtcgataa ggctattggt gtttatggct
6061 ctcttggtcg tcagactgat gggccc
//
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